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Absolutely. In fact, if you use the three memory slots you must use this configuration to take full advantage of dual channel. Slot 1 & 3 work as a single DIMM working in parallel with slot 2; thus both slots 1 & 3 should have the same memory size and configuration (number of chips per DIMM) and slot 3 should be the same size as slot 1 plus slot 3.
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